This method includes preparation of the animal for real time imaging, 2D and 3D image acquisition, and post-acquisition analysis of tumors volumes. The new and upgraded Vevo 3100 ultrasound system allows us to noninvasively detect and measure abnormalities as well as to compare to normal tissue, thereby identifying the magnitude of the abnormalities. Post acquisition analysis on ultrasound scans is offered with the Vevo 3100 Analysis software installed on a dedicated OPTIC computer for qualitative and quantitative assessments and measurements.